Journal: Molecular Cell

Article Title: An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress

doi: 10.1016/j.molcel.2021.06.017

Figure Lengend Snippet:

Article Snippet: The pSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid was provided by Feng Zhang via Addgene (plasmid #62988) and described in .

Techniques: Transduction, Virus, Recombinant, Fluorescence, Multiplex sample analysis, Protease Inhibitor, Transfection, Plasmid Preparation, Software

Journal: eLife

Article Title: Maf and Mafb control mouse pallial interneuron fate and maturation through neuropsychiatric disease gene regulation

doi: 10.7554/eLife.54903

Figure Lengend Snippet: ( A ) Heatmap of MGE IN marker genes for cluster 1 Sst - and Sst + INs. ( B–D’’ ) Lower magnification fluorescent images of multiplex RNA in situ hybridization for Mef2c ( B–B’’ ), Sp9 ( C–C’’ ) and Igfbp4 ( D–D’’ ). ( E–G’’’ ) Higher magnification fluorescent images of multiplex RNA in situ hybridization for Mef2c ( E–E’’’ ), Sp9 ( F–F’’’ ) and Igfbp4 ( G–G’’’ ). White arrows point to INs that are marker ( Mef2c , Sp9 or Igfbp4 ) and tdTomato positive but Sst negative. Violin plots showing the normalized expression value (Y-axis) of each cell analyzed in each group for Mef2c ( H ), Sp9 ( J ) and Igfbp4 ( L ). Quantification of the percentage of tdTomato + ; Sst - and tdTomato + ; Sst + INs in the neocortex and in the hippocampus that are either Mef2c + ( I ), Sp9 + ( K ) or Igfbp4 + ( M ). Scale bar in ( B ) = 100 um, ( B’’ ) = 200 um and ( G ) = 25 um. 2 WTs and multiple brain sections per animal were used for quantification. For statistical analysis, multiple independent t-tests without same standard deviation assumption were conducted to compare the expression of each gene in each brain region. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Article Snippet: Commercial assay or kit , Mm- Igfbp4 , ACD Bio , Cat #425711 , Follow the manufacturer’s instruction and used it with the RNAscope multiplex fluorescent kit (version 2).

Techniques: Marker, Multiplex Assay, RNA In Situ Hybridization, Expressing, Standard Deviation

Journal: eLife

Article Title: Maf and Mafb control mouse pallial interneuron fate and maturation through neuropsychiatric disease gene regulation

doi: 10.7554/eLife.54903

Figure Lengend Snippet: Higher magnification of HINs that are marker positive [ Igfbp4 (A-A’’’), Sp9 (B-B’’’), Mef2c (C-C’’’), Tcf12 (D-D’’’), Elmo1 (E-E’’’) and Arl4d (F-F’’’)] that are either in the Sst -lineage or in the proposed immature PV-lineage. Circle: representative cell that is Sst + In but has the proposed PV marker; Triangle: representative cell that is proposed to be PV candidate IN; Square: representative cell that is Sst + IN. Scale bar in ( A ) = 25 um.

Article Snippet: Commercial assay or kit , Mm- Igfbp4 , ACD Bio , Cat #425711 , Follow the manufacturer’s instruction and used it with the RNAscope multiplex fluorescent kit (version 2).

Techniques: Marker

Journal: eLife

Article Title: Maf and Mafb control mouse pallial interneuron fate and maturation through neuropsychiatric disease gene regulation

doi: 10.7554/eLife.54903

Figure Lengend Snippet:

Article Snippet: Commercial assay or kit , Mm- Igfbp4 , ACD Bio , Cat #425711 , Follow the manufacturer’s instruction and used it with the RNAscope multiplex fluorescent kit (version 2).

Techniques: Staining, RNAscope, Multiplex Assay

Journal: bioRxiv

Article Title: Stress vesicles are induced by acute mechanical force and precede the commitment of epidermal stem cells to terminal differentiation

doi: 10.1101/2022.09.29.510204

Figure Lengend Snippet: (A) Representative planes from serial optical sectioning of the live mouse epidermis showing the calcium gradient from the basal to suprabasal layers. (B) Representative RNA-scope staining indicating the efficiency of Piezo1 gene deletion epidermal cells. Scale bars, 10 μm. (C) Maximum intensity projection of calcium signals from timelapse imaging of control and Piezo1 cKO mouse skin under normal conditions. Individual frames were pseudocolored and projected into a single image to illustrate areas of persistent calcium signal (white). Scale bars, 50 μm. (D) Quantification of calcium signals from (C). (E) Representative images of in vivo imaging of calcium flux after Yoda1 (Piezo1 activator) treatment. (F) Representative images of stress vesicle formation after treatment with PBS Yoda1 or GsMTx4 (Piezo1 inhibitor). Scale bars, 20 μm. (G) Quantification of the number of cells with vesicle formation after the treatment of PBS, Yoda1, and GsMTx4. n= 5 mice for each group, p = 0.004 (***) and 0.9940 (ns).

Article Snippet: RNAscope experiment was performed by using the RNAscope Multiplex Fluorescent Reagent Kit, version 2 (#323100, Advanced Cell Diagnostic, Newark, CA) with Murine Piezo1 and K14 specific probe (Advanced Cell Diagnostics).

Techniques: Staining, Imaging, In Vivo Imaging

Journal: bioRxiv

Article Title: Stress vesicles are induced by acute mechanical force and precede the commitment of epidermal stem cells to terminal differentiation

doi: 10.1101/2022.09.29.510204

Figure Lengend Snippet: (A) Schematic of experimental design to conditionally ablate expression of Piezo1 in basal epidermal cells and observe the effect on stress vesicle formation. (B) Representative images of basal epidermal cells, from control and Piezo1 conditional knockout (cKO) mouse back skin, visualized before and right after mechanical force application. Scale bars, 10 μm. (C) Quantification of the fraction of basal epidermal cells with stress vesicles. n= 5 pairs of control and Piezo1 cKO mice, p < 0.0001. (D) Representative images of calcium signals at the plane of basal layer of the skin, from control and Piezo1 cKO mice, taken immediately after mechanical force application. Scale bars, 20 μm. (E) Quantification of calcium dynamics in 3 representative basal epidermal cells from Control and Piezo1 cKO mice under stressed conditions.

Article Snippet: RNAscope experiment was performed by using the RNAscope Multiplex Fluorescent Reagent Kit, version 2 (#323100, Advanced Cell Diagnostic, Newark, CA) with Murine Piezo1 and K14 specific probe (Advanced Cell Diagnostics).

Techniques: Expressing, Knock-Out

Journal: bioRxiv

Article Title: Stress vesicles are induced by acute mechanical force and precede the commitment of epidermal stem cells to terminal differentiation

doi: 10.1101/2022.09.29.510204

Figure Lengend Snippet: (A) Examples of mouse back skin imaged by brightfield (top panel) and two-photon (lower panel) microscopy after 10 min of applied negative pressure. Scale bars, 100 μm. (B) Quantification of the number of blisters on control and Piezo1 cKO back skin after after 10 min of applied negative pressure. n= 7 pairs of mice, p < 0.0001.

Article Snippet: RNAscope experiment was performed by using the RNAscope Multiplex Fluorescent Reagent Kit, version 2 (#323100, Advanced Cell Diagnostic, Newark, CA) with Murine Piezo1 and K14 specific probe (Advanced Cell Diagnostics).

Techniques: Microscopy